Sci250 week 1 chapter 3 staining lab quiz
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SCI250 Week 1 Chapter 3 Staining Lab Quiz
Section: Multiple Choice (highlight, bold, or underline your response)
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Which of the following stains is used frequently to identify Mycobacterium and other bacteria whose cell walls contain high amounts of lipids?
- Gram stain
- Schaeffer-Fulton stain
- Acid-fast stain
- Lipidialar stain
- Spore Stain
2. Which of the following stains is used to classify microorganisms based on their cell wall content?
- Capsular stain
- Gram stain
- Spore stain
- Negative stain
- Methylene blue
.3. In the Gram stain, Gram-positive bacteria stain with a _________ color due to the incorporation of the _________ dye into their cell wall.
- purple, safranin
- purple, crystal violet
- red, safranin
- red, crystal violet
4.What is the function of the mordant iodine in a Gram staining procedure?
- A mordant causes the crystal violet to run.
- A mordant fixes the bacteria to the slide.
- A mordant keeps dye attached to an object.
- All of the above
5.What is the order of reagents used in the Gram stain?
- crystal violet, iodine, safranin, alcohol
- alcohol, crystal violet, iodine, safranin
- iodine, crystal violet, safranin, alcohol
- crystal violet, iodine, alcohol, safranin
- crystal violet, safranin, alcohol, iodine
6. Following a Gram stain you observe clear, glassy areas inside the bacterial cells. What does this suggest? Which stain would you use to confirm this?
- A simple stain
- A flagellar stain
- Another Gram stain
- An endospore stain
- An acid-fast stain
7.The presence of a capsule around bacterial cells usually indicates their increased disease-causing potential and resistance to disinfection. Capsules are generally viewed by ________
- spore staining.
- scanning electron microscopy.
- Gram staining.
- Ziehl-Neelsen staining.
- negative staining.
Section: True or False
8.Fluorescent microscopes use an ultraviolet (UV) light source and fluorochromes as stains.
9.Older, pure cultures of Gram-positive organisms can appear red when Gram stained.
Section: Matching
10.Use of two or more dyes to differentiate among bacterial species or to distinguish various structures of an organism; for example, the Gram stain.
- Differential stain
- Flagellar stain
- Gram stain
- Negative stain
- Schaeffer-Fulton spore stain
- Simple stain
- Ziehl-Neelsen acid-fast stain
- Cationic dye (also called basic dye)
11.A technique for observing flagella by coating the surfaces of flagella with a dye or a metal such as silver.
- Differential stain
- Flagellar stain
- Gram stain
- Negative stain
- Schaeffer-Fulton spore stain
- Simple stain
- Ziehl-Neelsen acid-fast stain
- Cationic dye (also called basic dye)
12.A differential stain used to make endospores easier to visualize.
- Differential stain
- Flagellar stain
- Gram stain
- Negative stain
- Schaeffer-Fulton spore stain
- Simple stain
- Ziehl-Neelsen acid-fast stain
- Cationic dye (also called basic dye)
13.A single dye used to reveal basic cell shapes and arrangements.
- Differential stain
- Flagellar stain
- Gram stain
- Negative stain
- Schaeffer-Fulton spore stain
- Simple stain
- Ziehl-Neelsen acid-fast stain
- Cationic dye (also called basic dye)
14.A differential stain for organisms that are not decolorized in alcohol, such as the bacteria that cause Hansen’s Disease (leprosy) and tuberculosis.
- Differential stain
- Flagellar stain
- Gram stain
- Negative stain
- Schaeffer-Fulton spore stain
- Simple stain
- Ziehl-Neelsen acid-fast stain
- Cationic dye (also called basic dye)
15.An ionic compound, used for staining bacteria, in which the positive ion imparts the color.
- Differential stain
- Flagellar stain
- Gram stain
- Negative stain
- Schaeffer-Fulton spore stain
- Simple stain
- Ziehl-Neelsen acid-fast stain
Cationic dye (also called basic dye)
Other CHEAP Resources:
SCI 250 v3 appendix C
http://www..com/content/sci-250-v3-appendix-c
SCI250 Week 1 Chapter 3 Staining Lab Quiz
http://www..com/content/sci250-week-1-chapter-3-staining-lab-quiz
SCI250 Week 5 Chapter 19 Integumentary System Quiz
http://www..com/content/sci250-week-5-chapter-19-integumentary-system-quiz
SCI250 Week 6 Chapter 21 Respiratory System Quiz
http://www..com/content/sci250-week-6-chapter-21-respiratory-system-quiz
SCI250 Week 7 Final Examination
http://www..com/content/sci250-week-7-final-examination
SCI250 Week 7 Chapter 24 Nervous System Quiz
http://www..com/content/sci250-week-7-chapter-24-nervous-system-quiz